ADIFAB Kits include ADIFAB, a storage buffer, a measuring buffer and a standard.
Catalog #: ADI-1-200
Unit Size: 0.2 mg
Catalog #: ADI-1-1000
Unit Size: 1.0 mg
Prices do not include shipping and taxes. ADIFAB is shipped on blue ice.
For plasma/serum, use ADIFAB2.
The assay can be used in a variety of biochemical and clinical applications including the determination of lipase activity, fatty acid binding to membranes and proteins, and serum unbound free fatty acid levels.
You can determine unbound free fatty acid levels in an aqueous solution with a simple, one step procedure that completes in approximately one minute: add a small amount of the ADIFAB reagent to a solution suspected of containing unbound free fatty acid and measure the resulting fluorescence. In the absence of unbound free fatty acids, the ADIFAB probe fluoresces in the blue at 432 nm. In the presence of unbound free fatty acids, the emission shifts to the green with a peak at 505 nm. The ratio of fluorescence at 505 nm and 432 nm allows the concentration of unbound free fatty acids to be determined without further calibration. For further details, refer to the protocols.
ADIFAB should be stored frozen upon receipt and should be protected from light. After ADIFAB is brought up in a solution, it should be refrigerated. The product is stable in a storage buffer at 4°C for >3 months.
Patent Number 5,470,714
ADIFAB does not contain any hazardous components above 1% or any carcinogens above 0.1% as defined in 29 CFR 1910.1200, the OSHA Hazard Communication Standard.
Intracellular FFAu levels measured with ADIFAB