Metabolomics:Probes for unbound free fatty acids using fluorescent fatty acid binding proteins

New Probes

FFA Sciences is developing new fluorescent probes using mutated fatty acid binding proteins. These probes reveal enhanced specificity for individual FFA and other metabolites and are now available from us.

FFA Specific Probes

PAL201: a high affinity probe with enhanced specificity for palmitate (16:0).

OAL1301: a high affinity probe with enhanced specificity for oleate (18:1).

These and other FFA specific probes have been used to determine the profile of different unbound FFA in mixtures of FFA in equilibrium with albumin as described in the following publication:

Fatty Acid-Specific Fluorescent Probes and Their Use in Resolving Mixtures of Unbound Free Fatty Acids in Equilibrium with Albumin Andrew H. Huber, J. Patrick Kampf, Thomas Kwan, Baolong Zhu, and Alan M. Kleinfeld Biochemistry; 2006; 45:14263-14274

For more information concerning the new probes, refer to their Protocols.

Bilirubin Specific Probe

BLL2401: a probe with a high degree of specificity for free (unbound) unconjugated bilirubin. This probe which does not respond to FFA reveals bilirubin specific quenching (excitation = 375 nm and emission = 525 nm) with a Kd for bilirubin at 22ºC of 22 nM.

For more information concerning the new bilirubin probe, refer to the Protocols.

Price Information

Our kits include a reagent and, at no additional cost, a storage buffer, a measuring buffer and 0.25 ml of an unbound free fatty acid standard.

Catalog #: PAL201-200 or OAL1301-200

Unit Size: 0.2 mg

Price: $300

Catalog #: PAL201-1000 or OAL1301-1000

Unit Size: 1.0 mg

Price: $1,000

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